Tyrosinase Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-0819R-Cy5.5-100uL |
| Product Name | Tyrosinase Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Cow, Sheep, Pig |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Tyrosinase |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 7299 |
| Subcellular location | Cytoplasm |
| Synonyms | LB24 AB; LB24-AB; Monophenol monooxygenase; SK29 AB; Tumor rejection antigen AB; LB24 AB; Monophenol monooxygenase; OCA1A; OCAIA; Oculocutaneous albinism IA; SK29 AB; SK29-AB; Tumor rejection antigen AB; TYR; tyrosinase oculocutaneous albinism IA; TYRO_HUMAN. |
| Background | Tyrosinase is the key enzyme for melanin,synthesis in mammalian melanocytes and has been considered to be a unique marker for the study of melanocyte differentiation. A cDNA library was constructed from poly(A)+ mRNA from mouse melanocytes and screened using anti- tyrosinase antiserum and oligonucleotide probes corresponding to amino acid sequence of tyrosinase. sequencing of some cDNA clones positive in these screenings gave a nucleotide sequence of 1838 nucleotides including a open reading frame of 1344 nucleotides that was found to correspond exactly to the amino acid sequence of the cyanogen bromide fragments of tyrosinase. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Tyrosinase Polyclonal Antibody, Cy5.5 Conjugated (BS-0819R-CY5-5) validated for, and at what dilution?
BS-0819R-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Tyrosinase Polyclonal Antibody, Cy5.5 Conjugated (BS-0819R-CY5-5) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Sheep, Pig.
How should BS-0819R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Tyrosinase Polyclonal Antibody, Cy5.5 Conjugated (BS-0819R-CY5-5)?
KLH conjugated synthetic peptide derived from human Tyrosinase
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-0819R-CY5-5)?
Cy5.5 on BS-0819R-CY5-5: excitation / emission 675nm/694nm.