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Granulin Polyclonal Antibody, Cy5 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human

Predicted Reactivity

  • Mouse
  • Rat
  • Dog
  • Cow
  • Pig
  • Horse
  • Rabbit
  • GuineaPig
Overview
Catalog # bs-0823R-Cy5-TR
Product Name Granulin Polyclonal Antibody, Cy5 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Specificity This antibody is specific for Granulin-6
Reactivity Human
Predicted Reactivity Mouse, Rat, Dog, Cow, Pig, Horse, Rabbit, GuineaPig
Specifications
Conjugation Cy5
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Granulin
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 2896
Swiss Prot P28799
Subcellular location Secreted
Synonyms GEP; GP88; PEPI; PGRN; CLN11; PCDGF; Granulins; Proepithelin; GRN
Background Granulins have possible cytokine-like activity. They may play a role in inflammation, wound repair, and tissue remodeling. Granulin-4 promotes proliferation of the epithelial cell line A431 in culture while granulin-3 acts as an antagonist to granulin-4, inhibiting the growth.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is Granulin Polyclonal Antibody, Cy5 Conjugated (bs-0823R-Cy5) validated for, and at what dilution?

bs-0823R-Cy5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Granulin Polyclonal Antibody, Cy5 Conjugated (bs-0823R-Cy5) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Pig, Horse, Rabbit, GuineaPig.

How should bs-0823R-Cy5 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Granulin Polyclonal Antibody, Cy5 Conjugated (bs-0823R-Cy5)?

KLH conjugated synthetic peptide derived from human Granulin

What are the excitation and emission wavelengths of the Cy5 conjugate (bs-0823R-Cy5)?

Cy5 on bs-0823R-Cy5: excitation / emission 625,650nm/670nm.