TTF1/NKX2-1 Polyclonal Antibody, RBITC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-0826R-RBITC-100uL |
| Product Name | TTF1/NKX2-1 Polyclonal Antibody, RBITC Conjugated |
| Applications |
WB
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human TTF-1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 7080 |
| Swiss Prot | P43699 |
| Subcellular location | Nucleus |
| Synonyms | BCH; BHC; NK-2; TEBP; TTF1; NKX2A; T/EBP; TITF1; TTF-1; NKX2.1; Homeobox protein Nkx-2.1; Homeobox protein NK-2 homolog A; Thyroid nuclear factor 1; Thyroid transcription factor 1; Thyroid-specific enhancer-binding protein; NKX2-1 |
| Background | Transcription factor that binds and activates the promoter of thyroid specific genes such as thyroglobulin, thyroperoxidase, and thyrotropin receptor. Crucial in the maintenance of the thyroid differentiation phenotype. May play a role in lung development and surfactant homeostasis. Activates the transcription of GNRHR and plays a role in enhancing the circadian oscillation of its gene expression. Represses the transcription of the circadian transcriptional repressor NR1D1 (By similarity). |
| Application Dilution | |
| WB | 1:300-5000 |
| IF | FCM1:20-100 |
Frequently asked questions
Which applications is TTF1/NKX2-1 Polyclonal Antibody, RBITC Conjugated (bs-0826R-RBITC) validated for, and at what dilution?
bs-0826R-RBITC is validated for WB (1:300-5000); FCM (1:20-100); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does TTF1/NKX2-1 Polyclonal Antibody, RBITC Conjugated (bs-0826R-RBITC) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-0826R-RBITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise TTF1/NKX2-1 Polyclonal Antibody, RBITC Conjugated (bs-0826R-RBITC)?
KLH conjugated synthetic peptide derived from human TTF-1
What are the excitation and emission wavelengths of the RBITC conjugate (bs-0826R-RBITC)?
RBITC on bs-0826R-RBITC: excitation / emission 540nm/625nm.