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Insulin Polyclonal Antibody, APC-Cy7 Conjugated

Applications

  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bs-0855R-APC-Cy7-100uL
Product Name Insulin Polyclonal Antibody, APC-Cy7 Conjugated
Applications
IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Mouse, Rat
Specifications
Conjugation APC-Cy7
Host Rabbit
Source Recombinant human insulin
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 3630
Subcellular location Secreted
Synonyms ILPR; INS; Insulin A chain; Insulin B chain; Insulin A chain; Insulin precursor; IRDN; Proinsulin; Proinsulin precursor; IDDM2; INS_HUMAN; MODY10.
Background Insulin is a pancreatic hormone that regulates glucose and is involved in the synthesis of protein and fat. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. Heterodimer of a B chain and an A chain linked by two disulfide bonds.Belongs to the insulin family. The insulin-link growth factors, IGF-I and IGF-II (also desinated somatomedin C and multiplication stimulating activator, respectvely), share approximatly 76% sequence identity and are 50% related to pro-insulin.IGF-I and IGF-II are nonglycosylated, single chain proteins of 70 and 76 amino acids in length, respectivelly. IGF-I functions as an autocrine regulator of growth in vaious, whereas the function of IGF-II is less well defined.
Application Dilution
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is Insulin Polyclonal Antibody, APC-Cy7 Conjugated (bs-0855R-APC-Cy7) validated for, and at what dilution?

bs-0855R-APC-Cy7 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Insulin Polyclonal Antibody, APC-Cy7 Conjugated (bs-0855R-APC-Cy7) react with?

Tested reactivity: Human, Mouse, Rat.

How should bs-0855R-APC-Cy7 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Insulin Polyclonal Antibody, APC-Cy7 Conjugated (bs-0855R-APC-Cy7)?

Recombinant human insulin

What are the excitation and emission wavelengths of the APC-Cy7 conjugate (bs-0855R-APC-Cy7)?

APC-Cy7 on bs-0855R-APC-Cy7: excitation / emission 650nm/780nm.

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