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Insulin Polyclonal Antibody, AbBy Fluor-647 Conjugated

Applications

  • WB
  • IF(IHC-P)

Reactivity

  • Human

Predicted Reactivity

  • Mouse
  • Rat
  • Pig
Overview
Catalog # bs-0862R-BF647
Product Name Insulin Polyclonal Antibody, AbBy Fluor-647 Conjugated
Applications
WB IF(IHC-P)
Reactivity Human
Predicted Reactivity Mouse, Rat, Pig
Specifications
Conjugation AbBy Fluor-647
Host Rabbit
Source KLH conjugated Insulin from porcine pancreas
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 397415
Swiss Prot P01315
Subcellular location Secreted
Synonyms Insulin; INS
Background Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200

Frequently asked questions

Which applications is Insulin Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-0862R-BF647) validated for, and at what dilution?

bs-0862R-BF647 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Insulin Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-0862R-BF647) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Pig.

How should bs-0862R-BF647 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Insulin Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-0862R-BF647)?

KLH conjugated Insulin from porcine pancreas

What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bs-0862R-BF647)?

AbBy Fluor-647 on bs-0862R-BF647: excitation / emission 650nm/665nm.