DEK Polyclonal Antibody, Cy5.5 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-0874R-Cy5.5-100uL |
| Product Name | DEK Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Chicken |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human DEK |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 7913 |
| Subcellular location | Nucleus |
| Synonyms | D6S231E; Dek; DEK gene; DEK oncogene; DEK oncogene DNA binding; DNA binding; DEK_HUMAN; OTTHUMP00000039357; Protein DEK. |
| Background | The DEK gene encodes a protein with one SAP domain. This protein binds to cruciform and superhelical DNA which then induces positive supercoils into closed circular DNA, and is also involved during mRNA processing in splice site selection. Chromosomal aberrations involving this region, increased expression of this gene, and the presence of against this protein are all associated with various diseases. Two transcript variants encoding different isoforms have been found for this gene. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is DEK Polyclonal Antibody, Cy5.5 Conjugated (BS-0874R-CY5-5) validated for, and at what dilution?
BS-0874R-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should BS-0874R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise DEK Polyclonal Antibody, Cy5.5 Conjugated (BS-0874R-CY5-5)?
KLH conjugated synthetic peptide derived from human DEK
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-0874R-CY5-5)?
Cy5.5 on BS-0874R-CY5-5: excitation / emission 675nm/694nm.