Recombinant GST protein, Tag free
Applications
Reactivity
| Overview | |
| Catalog # | bs-0883p-100ug |
| Product Name | Recombinant GST protein, Tag free |
| Applications |
ELISA
|
| Reactivity | Others |
| Specifications | |
| Conjugation | Unconjugated |
| Source | Recombinant GST protein, Tag free protein is expressed in E.coli with Tag free. It contains the amino acid sequence of 1-211/211. |
| Storage Buffer | 0.01M TBS(pH7.4) |
| Storage Condition | The product should be stored at -70°C or -20°C. |
| Target | |
| Swiss Prot | N/A |
| Background | Genetic engineers have used glutathione S-transferase to create the GST gene fusion system. This system is used to purify and detect proteins of interest. In a GST gene fusion system, the GST sequence is incorporated into an expression vector alongside the gene sequence encoding the protein of interest. Induction of protein expression from the vector's promoter results in expression of a fusion protein: the protein of interest fused to the GST protein. This GST-fusion protein can then be purified from cells via its high affinity for glutathione. GST is commonly used to create fusion proteins. The tag has the size of 22amino acids(roughly 26 KDa), which, compared to other tags like the Myc- or the FLAG-tag, is quite big. However, many commercially-available sources of GST-tagged plasmids include athrombindomain for cleavage of the GST tag during protein purification. |
Frequently asked questions
Which applications is Recombinant GST protein, Tag free (bs-0883P) validated for, and at what dilution?
bs-0883P is validated for ELISA.
How should bs-0883P be stored?
The product should be stored at -70°C or -20°C. Storage buffer: 0.01M TBS(pH7.4)
How is Recombinant GST protein, Tag free (bs-0883P) produced?
Recombinant GST protein, Tag free protein is expressed in E.coli with Tag free. It contains the amino acid sequence of 1-211/211.