phospho-BAD (Ser128) Polyclonal Antibody, PE Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-0893R-PE |
| Product Name | phospho-BAD (Ser128) Polyclonal Antibody, PE Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Specificity | This phophorylation site is homologous to that of Ser129 in Rat. |
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | PE |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from mouse BAD around the phosphorylation site of Ser128 |
| Modification Site | Ser128 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 12015 |
| Swiss Prot | Q61337 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | Bbc2; AI325008; Bcl2-associated agonist of cell death; BAD; Bcl-2-binding component 6; Bcl-xL/Bcl-2-associated death promoter; Bcl2 antagonist of cell death; Bbc6 |
| Background | Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2. Appears to act as a link between growth factor receptor signaling and the apoptotic pathways. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | |
| IF(IHC-F) | |
| IF(ICC) | |
Frequently asked questions
Which applications is phospho-BAD (Ser128) Polyclonal Antibody, PE Conjugated (bs-0893R-PE) validated for, and at what dilution?
bs-0893R-PE is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-BAD (Ser128) Polyclonal Antibody, PE Conjugated (bs-0893R-PE) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-0893R-PE be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-BAD (Ser128) Polyclonal Antibody, PE Conjugated (bs-0893R-PE)?
KLH conjugated synthetic phosphopeptide derived from mouse BAD around the phosphorylation site of Ser128
What are the excitation and emission wavelengths of the PE conjugate (bs-0893R-PE)?
PE on bs-0893R-PE: excitation / emission 496,564nm/578nm.