BrdU(Proliferation Marker) Polyclonal Antibody, AbBy Fluor-488 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-0917R-BF488 |
| Product Name | BrdU(Proliferation Marker) Polyclonal Antibody, AbBy Fluor-488 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Species independent |
| Specifications | |
| Conjugation | AbBy Fluor-488 |
| Host | Rabbit |
| Source | BrdU conjugated to KLH |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Nucleus |
| Synonyms | Bromodeoxyuridine; Bromodeoxyuridine; 5-Bromo-2_-deoxyuridine; 5-BrdU; proliferation Marker. |
| Background | Bromodeoxyuridine is a synthetic nucleoside that is an analogue of thymidine. BrdU is incorporated into DNA during S-phase of a cell cycle. It is commonly used in the detection of proliferating cells in living tissues |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is BrdU(Proliferation Marker) Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-0917R-BF488) validated for, and at what dilution?
bs-0917R-BF488 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does BrdU(Proliferation Marker) Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-0917R-BF488) react with?
Tested reactivity: Species independent.
How should bs-0917R-BF488 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise BrdU(Proliferation Marker) Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-0917R-BF488)?
BrdU conjugated to KLH
What are the excitation and emission wavelengths of the AbBy Fluor-488 conjugate (bs-0917R-BF488)?
AbBy Fluor-488 on bs-0917R-BF488: excitation / emission 499nm/519nm.