Mafa Polyclonal Antibody, APC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-0924R-APC-100uL |
| Product Name | Mafa Polyclonal Antibody, APC Conjugated |
| Applications |
WB
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | APC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Mafa |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 389692 |
| Subcellular location | Nucleus |
| Synonyms | Mafa homolog; V-maf musculoaponeurotic fibrosarcoma oncogene homolog A;Pancreatic beta-cell-specic transcriptional activator;avianV-maf musculoaponeurotic fibrosarcoma oncogene homolog A; MAFA_MOUSE. |
| Background | Insulin gene expression is regulated by several islet-enriched transcription factors. However, MAFA is the only beta cell-specific activator. MAFA selectively induces endogenous insulin transcription in non-beta cells. MAFA was also first detected in the insulin-producing cells formed during the second and predominant phase of beta cell differentiation, and absent in the few insulin-positive cells found in Nkx6.1(-/-) pancreata, which lack the majority of second-phase beta cells. These results demonstrate that MAFA is a potent insulin activator that is likely to function downstream of Nkx6.1 during islet insulin-producing cell development. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF | IHC-P1:50-200 |
Frequently asked questions
Which applications is Mafa Polyclonal Antibody, APC Conjugated (bs-0924R-APC) validated for, and at what dilution?
bs-0924R-APC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Mafa Polyclonal Antibody, APC Conjugated (bs-0924R-APC) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-0924R-APC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Mafa Polyclonal Antibody, APC Conjugated (bs-0924R-APC)?
KLH conjugated synthetic peptide derived from human Mafa
What are the excitation and emission wavelengths of the APC conjugate (bs-0924R-APC)?
APC on bs-0924R-APC: excitation / emission 650nm/660nm.