Collagen 3 Polyclonal Antibody, AbBy Fluor-647 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-0948r-bf647-100uL |
| Product Name | Collagen 3 Polyclonal Antibody, AbBy Fluor-647 Conjugated |
| Applications |
WB
|
| Reactivity | Human |
| Predicted Reactivity | Dog, Cow, Chicken |
| Specifications | |
| Conjugation | AbBy Fluor-647 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Collagen alpha 1(III) chain |
| Immunogen Range | 801-900/1466 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1281 |
| Swiss Prot | P02461 |
| Subcellular location | Secreted, Extracellular matrix |
| Synonyms | EDS4A; Collagen alpha-1(III) chain; COL3A1; Collagen III |
| Background | Collagen type III occurs in most soft connective tissues along with type I collagen. Involved in regulation of cortical development. Is the major ligand of GPR56 in the developing brain and binding to GPR56 inhibits neuronal migration and activates the RhoA pathway by coupling GPR56 to GNA13 and possibly GNA12. |
| Application Dilution | |
| WB | 1:500-2000 |
Frequently asked questions
Which applications is Collagen 3 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-0948R-BF647) validated for, and at what dilution?
bs-0948R-BF647 is validated for WB (1:500-2000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Collagen 3 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-0948R-BF647) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Chicken.
How should bs-0948R-BF647 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Collagen 3 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-0948R-BF647)?
KLH conjugated synthetic peptide derived from human Collagen alpha 1(III) chain Immunogen range: 801-900/1466.
What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bs-0948R-BF647)?
AbBy Fluor-647 on bs-0948R-BF647: excitation / emission 650nm/665nm.