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Collagen 3 Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 20min; Antibody incubation with Collagen III Polyclonal Antibody, Unconjugated (bs-0948R) at 1:200 overnight at 4\u00b0C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded human colon cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Collagen III Polyclonal Antibody, Unconjugated (bs-0948R) at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining.

Applications

  • WB
  • Flow-Cyt
  • ICC/IF

Reactivity

  • Human

Predicted Reactivity

  • Dog
  • Cow
  • Chicken
Overview
Catalog # bs-0948r
Product Name Collagen 3 Polyclonal Antibody
Applications WB, Flow-Cyt, ICC/IF
Reactivity Human
Predicted Reactivity Dog, Cow, Chicken
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Collagen alpha 1(III) chain
Clonality Polyclonal
Clone # #REF!
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 1281
Swiss Prot P02461
Subcellular location Secreted, Extracellular matrix
Synonyms EDS4A; Collagen alpha-1(III) chain; COL3A1
Background Collagen type III occurs in most soft connective tissues along with type I collagen. Involved in regulation of cortical development. Is the major ligand of GPR56 in the developing brain and binding to GPR56 inhibits neuronal migration and activates the RhoA pathway by coupling GPR56 to GNA13 and possibly GNA12.
Application Dilution
WB 1:500-2000
Flow-Cyt
ICC/IF

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