Phospho-HP1 alpha (Tyr37) Polyclonal Antibody
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-0955R-100uL |
| Product Name | Phospho-HP1 alpha (Tyr37) Polyclonal Antibody |
| Applications |
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human CBX5 around the phosphorylation site of Tyr37 |
| Modification Site | Tyr37 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 23468 |
| Subcellular location | Nucleus |
| Synonyms | Antigen p25; Cbx 5; Cbx5; CBX5_HUMAN; CG8409; Chromobox homolog 5 Drosophila HP1 alpha; Chromobox homolog 5 HP1 alpha homolog Drosophila; Chromobox homolog 5 HP1 alpha homolog, Drosophila; chromobox homolog 5; HP1 alpha homolog; Chromobox protein homolog 5; Chromobox protein homologue 5; Heterochromatin protein 1 alpha; Heterochromatin protein 1; Heterochromatin protein 1; HP 1; HP 1A; HP1 alpha homolog; HP1; HP1A; HP1Hs alpha; Suvar205; HP1a; HP1Hs-alpha; HP1 alpha; Antigen p25; Heterochromatin protein 1 homolog alpha. |
| Background | This gene encodes a highly conserved nonhistone protein, which is a member of the heterochromatin protein family. The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The encoded product is involved in the formation of functional kinetochore through interaction with essential kinetochore proteins. The gene has a pseudogene located on chromosome 3. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq]. |
| Application Dilution | |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
| IHC-F | 1:100-500 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Phospho-HP1 alpha (Tyr37) Polyclonal Antibody (bs-0955R) validated for, and at what dilution?
bs-0955R is validated for ELISA (1:500-1000); IHC-P (1:200-400); IHC-F (1:100-500); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-0955R be stored?
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-HP1 alpha (Tyr37) Polyclonal Antibody (bs-0955R)?
KLH conjugated synthetic phosphopeptide derived from human CBX5 around the phosphorylation site of Tyr37