TFRC Polyclonal Antibody, HRP Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-0988R-HRP-100uL |
| Product Name | TFRC Polyclonal Antibody, HRP Conjugated |
| Applications |
WB
IHC-P
IHC-F
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat, Dog, Cow, Pig, Horse, Guinea Pig |
| Specifications | |
| Conjugation | HRP |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Transferrin receptor |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 7037 |
| Swiss Prot | P02786 |
| Subcellular location | Cell membrane |
| Synonyms | T9; TR; TFR; p90; CD71; TFR1; TRFR; Transferrin receptor protein 1; TFRC |
| Background | Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. |
| Application Dilution | |
| WB | 1:300-5000 |
| IHC-P | ELISA1:500-1000 |
| IHC-F | IHC-P1:200-400 |
Frequently asked questions
Which applications is TFRC Polyclonal Antibody, HRP Conjugated (bs-0988R-HRP) validated for, and at what dilution?
bs-0988R-HRP is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does TFRC Polyclonal Antibody, HRP Conjugated (bs-0988R-HRP) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Cow, Pig, Horse, Guinea Pig.
How should bs-0988R-HRP be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise TFRC Polyclonal Antibody, HRP Conjugated (bs-0988R-HRP)?
KLH conjugated synthetic peptide derived from human Transferrin receptor