TFRC Polyclonal Antibody

Image provided by One World Lab validation program. U138 cells probed with Rabbit Anti-Transferrin receptor Polyclonal Antibody (bs-0988R) at 1:50 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.

Raji cells probed with \\tTransferrin receptor Polyclonal Antibody, Unconjugated (bs-0988R) at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

K562 cells were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Transferrin receptor Polyclonal Antibody(bs-0988R)at 1:100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Paraformaldehyde-fixed, paraffin embedded (human liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (TFRC ) Polyclonal Antibody, Unconjugated (bs-0988R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.