DDX58 Polyclonal Antibody, HRP Conjugated

Applications

  • WB
  • IHC-P

Reactivity

  • Mouse

Predicted Reactivity

  • Human
  • Rat
  • Dog
  • Cow
  • Pig
Overview
Catalog # bs-0993R-HRP
Product Name DDX58 Polyclonal Antibody, HRP Conjugated
Applications WB, IHC-P
Reactivity Mouse
Predicted Reactivity Human, Rat, Dog, Cow, Pig
Specifications
Conjugation HRP
Host Rabbit
Source KLH conjugated synthetic peptide derived from human DDX58
Immunogen Range 201-300/925
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles
Target
Gene ID 23586
Swiss Prot O95786
Subcellular location Cytoplasm
Synonyms RIGI; RIG-I; RLR-1; SGMRT2; Probable ATP-dependent RNA helicase DDX58; DEAD box protein 58; RIG-I-like receptor 1; Retinoic acid-inducible gene 1 protein; RIG-1; Retinoic acid-inducible gene I protein; DDX58
Background Innate immune receptor which acts as a cytoplasmic sensor of viral nucleic acids and plays a major role in sensing viral infection and in the activation of a cascade of antiviral responses including the induction of type I interferons and proinflammatory cytokines. Its ligands include: 5'-triphosphorylated ssRNA and dsRNA and short dsRNA (<1 kb in length). In addition to the 5'-triphosphate moiety, blunt-end base pairing at the 5'-end of the RNA is very essential. Overhangs at the non-triphosphorylated end of the dsRNA RNA have no major impact on its activity. A 3'overhang at the 5'triphosphate end decreases and any 5'overhang at the 5' triphosphate end abolishes its activity. Upon ligand binding it associates with mitochondria antiviral signaling protein (MAVS/IPS1) which activates the IKK-related kinases: TBK1 and IKBKE which phosphorylate interferon regulatory factors: IRF3 and IRF7 which in turn activate transcription of antiviral immunological genes, including interferons (IFNs); IFN-alpha and IFN-beta. Detects both positive and negative strand RNA viruses including members of the families Paramyxoviridae: Human respiratory syncytial virus and measles virus (MeV), Rhabdoviridae: vesicular stomatitis virus (VSV), Orthomyxoviridae: influenza A and B virus, Flaviviridae: Japanese encephalitis virus (JEV), hepatitis C virus (HCV), dengue virus (DENV) and west Nile virus (WNV). It also detects rotavirus and reovirus. Also involved in antiviral signaling in response to viruses containing a dsDNA genome such as Epstein-Barr virus (EBV). Detects dsRNA produced from non-self dsDNA by RNA polymerase III, such as Epstein-Barr virus-encoded RNAs (EBERs). May play important roles in granulocyte production and differentiation, bacterial phagocytosis and in the regulation of cell migration.
Application Dilution
WB 1:300-5000
IHC-P 1:200-400