CTLA4 Polyclonal Antibody, FITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-10006R-FITC |
| Product Name | CTLA4 Polyclonal Antibody, FITC Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
|
| Reactivity | Human, Mouse, Rat |
| Predicted Reactivity | Dog, Cow, Sheep, Rabbit |
| Specifications | |
| Conjugation | FITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CTLA-4/CD152 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1493 |
| Swiss Prot | P16410 |
| Subcellular location | Cell membrane, Extracellular matrix |
| Synonyms | CD; GSE; GRD4; CD152; CTLA-4; IDDM12; CELIAC3; Cytotoxic T-lymphocyte protein 4; Cytotoxic T-lymphocyte-associated antigen 4; CTLA4 |
| Background | Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is CTLA4 Polyclonal Antibody, FITC Conjugated (bs-10006R-FITC) validated for, and at what dilution?
bs-10006R-FITC is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CTLA4 Polyclonal Antibody, FITC Conjugated (bs-10006R-FITC) react with?
Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Sheep, Rabbit.
How should bs-10006R-FITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CTLA4 Polyclonal Antibody, FITC Conjugated (bs-10006R-FITC)?
KLH conjugated synthetic peptide derived from human CTLA-4/CD152
What are the excitation and emission wavelengths of the FITC conjugate (bs-10006R-FITC)?
FITC on bs-10006R-FITC: excitation / emission 494nm/518nm.