EGFR Polyclonal Antibody, Biotin Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-10007R-Biotin-100uL |
| Product Name | EGFR Polyclonal Antibody, Biotin Conjugated |
| Applications |
WB
IHC-P
IHC-F
|
| Reactivity | Human, Mouse, Rat, Bovine, Pig |
| Specifications | |
| Conjugation | Biotin |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human EGFR |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C for 12 months. |
| Target | |
| Gene ID | 1956 |
| Subcellular location | Extracellular |
| Synonyms | EGFR; ERBB; ERBB1; HER1; mENA; PIG61; Avian erythroblastic leukemia viral verbb oncogene homolog; EGF R; EGFR; Epidermal growth factor receptor; erbb 1; Erbb; Erbb1; mENA; Oncogene ERBB; Receptor tyrosine protein kinase ErbB1; Urogastrone; wa2; Wa5 |
| Background | Protein kinases are enzymes that transfer a phosphate group from a phosphate donor onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. The protein kinase family is one of the largest families of proteins in eukaryotes, classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. Epidermal Growth factor receptor (EGFR) is the prototype member of the type 1 receptor tyrosine kinases. EGFR overexpression in tumors indicates poor prognosis and is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma. |
| Application Dilution | |
| WB | 1:300-5000 |
| IHC-P | 1:200-400 |
| IHC-F | |
Frequently asked questions
Which applications is EGFR Polyclonal Antibody, Biotin Conjugated (bs-10007R-Biotin) validated for, and at what dilution?
bs-10007R-Biotin is validated for WB (1:300-5000); IHC-P (1:200-400). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does EGFR Polyclonal Antibody, Biotin Conjugated (bs-10007R-Biotin) react with?
Tested reactivity: Human, Mouse, Rat, Bovine, Pig.
How should bs-10007R-Biotin be stored?
Store at -20C for 12 months. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise EGFR Polyclonal Antibody, Biotin Conjugated (bs-10007R-Biotin)?
KLH conjugated synthetic peptide derived from human EGFR