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Occludin Polyclonal Antibody, APC Conjugated

Applications

  • WB
  • FCM

Reactivity

  • Human

Predicted Reactivity

  • Mouse
  • Rat
  • Dog
  • Cow
  • Pig
  • Horse
  • GuineaPig
Overview
Catalog # bs-10011R-APC
Product Name Occludin Polyclonal Antibody, APC Conjugated
Applications
WB FCM
Reactivity Human
Predicted Reactivity Mouse, Rat, Dog, Cow, Pig, Horse, GuineaPig
Specifications
Conjugation APC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Occludin
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 100506658
Swiss Prot Q16625
Subcellular location Cell membrane
Synonyms Occludin, Occludin-1; Occludin1; Occludin 1; FLJ08163; BLCPMG; FLJ18079; FLJ77961; FLJ94056; MGC34277; Occludin; OCLN; OCLN_HUMAN; Tight junction protein occludin.
Background May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier.
Application Dilution
WB 1:300-5000
FCM 1:20-100

Frequently asked questions

Which applications is Occludin Polyclonal Antibody, APC Conjugated (bs-10011R-APC) validated for, and at what dilution?

bs-10011R-APC is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Occludin Polyclonal Antibody, APC Conjugated (bs-10011R-APC) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Pig, Horse, GuineaPig.

How should bs-10011R-APC be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Occludin Polyclonal Antibody, APC Conjugated (bs-10011R-APC)?

KLH conjugated synthetic peptide derived from human Occludin

What are the excitation and emission wavelengths of the APC conjugate (bs-10011R-APC)?

APC on bs-10011R-APC: excitation / emission 650nm/660nm.