Occludin Polyclonal Antibody, RBITC Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-10011R-RBITC |
| Product Name | Occludin Polyclonal Antibody, RBITC Conjugated |
| Applications |
WB
FCM
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Cow, Pig, Horse, GuineaPig |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Occludin |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 100506658 |
| Swiss Prot | Q16625 |
| Subcellular location | Cell membrane |
| Synonyms | Occludin, Occludin-1; Occludin1; Occludin 1; FLJ08163; BLCPMG; FLJ18079; FLJ77961; FLJ94056; MGC34277; Occludin; OCLN; OCLN_HUMAN; Tight junction protein occludin. |
| Background | May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is Occludin Polyclonal Antibody, RBITC Conjugated (bs-10011R-RBITC) validated for, and at what dilution?
bs-10011R-RBITC is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Occludin Polyclonal Antibody, RBITC Conjugated (bs-10011R-RBITC) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Pig, Horse, GuineaPig.
How should bs-10011R-RBITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Occludin Polyclonal Antibody, RBITC Conjugated (bs-10011R-RBITC)?
KLH conjugated synthetic peptide derived from human Occludin
What are the excitation and emission wavelengths of the RBITC conjugate (bs-10011R-RBITC)?
RBITC on bs-10011R-RBITC: excitation / emission 540nm/625nm.