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E2F2 Polyclonal Antibody

Applications

  • IHC-P
  • IF(IHC-P)

Reactivity

  • Human
  • Mouse
  • Rat
  • Dog
  • Pig
Overview
Catalog # bs-1001r-100uL
Product Name E2F2 Polyclonal Antibody
Applications
IHC-P IF(IHC-P)
Specificity High sequence similarity to E2F3. Expected to cross react to E2F3.
Reactivity Human, Mouse, Rat, Dog, Pig
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human E2F2
Immunogen Range 100-150/443
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C for 12 months.
Target
Gene ID 1870
Swiss Prot Q14209
Subcellular location Nucleus
Synonyms E2F-2; E2F2; E2F2_HUMAN; Transcription factor E2F2; E2F transcription factor 2
Background
Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from g1 to s phase. E2F2 binds specifically to RB1 in a cell-cycle dependent manner.
Application Dilution
IHC-P 1:200-400
IF(IHC-P) 1:50-200

Frequently asked questions

Which applications is E2F2 Polyclonal Antibody (bs-1001R) validated for, and at what dilution?

bs-1001R is validated for IHC-P (1:200-400); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does E2F2 Polyclonal Antibody (bs-1001R) react with?

Tested reactivity: Human, Mouse, Rat, Dog, Pig.

How should bs-1001R be stored?

Store at -20°C for 12 months. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise E2F2 Polyclonal Antibody (bs-1001R)?

KLH conjugated synthetic peptide derived from human E2F2 Immunogen range: 100-150/443.

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