E2F2 Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-1001r-cy3-100uL |
| Product Name | E2F2 Polyclonal Antibody, Cy3 Conjugated |
| Applications |
IF(IHC-P)
|
| Specificity | High sequence similarity to E2F3. Expected to cross react to E2F3. |
| Reactivity | Human, Mouse, Rat, Dog, Pig |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human E2F2 |
| Immunogen Range | 100-150/443 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1870 |
| Swiss Prot | Q14209 |
| Subcellular location | Nucleus |
| Synonyms | E2F-2; E2F2; E2F2_HUMAN; Transcription factor E2F2; E2F transcription factor 2 |
| Background | Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from g1 to s phase. E2F2 binds specifically to RB1 in a cell-cycle dependent manner. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is E2F2 Polyclonal Antibody, Cy3 Conjugated (bs-1001R-Cy3) validated for, and at what dilution?
bs-1001R-Cy3 is validated for IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does E2F2 Polyclonal Antibody, Cy3 Conjugated (bs-1001R-Cy3) react with?
Tested reactivity: Human, Mouse, Rat, Dog, Pig.
How should bs-1001R-Cy3 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise E2F2 Polyclonal Antibody, Cy3 Conjugated (bs-1001R-Cy3)?
KLH conjugated synthetic peptide derived from human E2F2 Immunogen range: 100-150/443.
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-1001R-Cy3)?
Cy3 on bs-1001R-Cy3: excitation / emission 512,550nm/570,615nm.