EIF5 Polyclonal Antibody, PerCP Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-10035R-PerCP-100uL |
| Product Name | EIF5 Polyclonal Antibody, PerCP Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Cow, Horse, Rabbit |
| Specifications | |
| Conjugation | PerCP |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human EIF5 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1983 |
| Swiss Prot | P55010 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | EIF-5; EIF-5A; Eukaryotic translation initiation factor 5; EIF5 |
| Background | Catalyzes the hydrolysis of GTP bound to the 40S ribosomal initiation complex (40S.mRNA.Met-tRNA[F].eIF-2.GTP) with the subsequent joining of a 60S ribosomal subunit resulting in the release of eIF-2 and the guanine nucleotide. The subsequent joining of a 60S ribosomal subunit results in the formation of a functional 80S initiation complex (80S.mRNA.Met-tRNA[F]). |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is EIF5 Polyclonal Antibody, PerCP Conjugated (bs-10035R-PerCP) validated for, and at what dilution?
bs-10035R-PerCP is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does EIF5 Polyclonal Antibody, PerCP Conjugated (bs-10035R-PerCP) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Cow, Horse, Rabbit.
How should bs-10035R-PerCP be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise EIF5 Polyclonal Antibody, PerCP Conjugated (bs-10035R-PerCP)?
KLH conjugated synthetic peptide derived from human EIF5
What are the excitation and emission wavelengths of the PerCP conjugate (bs-10035R-PerCP)?
PerCP on bs-10035R-PerCP: excitation / emission 482nm/677nm.