Netrin 4 Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-10065R-Cy3-100uL |
| Product Name | Netrin 4 Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF
|
| Reactivity | Others |
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Netrin 4 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 59277 |
| Subcellular location | Secreted, Cell membrane, Extracellular matrix |
| Synonyms | Beta netrin; Beta-netrin; Hepar derived netrin like protein; Hepar-derived netrin-like protein; NET4_HUMAN; Netrin-4; Netrin4; NTN 4; NTN4. |
| Background | Netrin proteins are a family of laminin-related secreted proteins that provide guidance signals for axonal growth and cell migration during development. Netrin signaling is dependent on the concentration of calcium outside the cell and the level of PKA activity. In axonal cells, a reduction in PKA activity converts the responsiveness of the axons to the netrin proteins, as the cells are repelled, rather than attracted, by the netrin gradient. Netrin-4 is related to the Laminin chains, and is therefore also designated -netrin. It is present in the basement membranes of the vasculature, lateral olfactory tract, kidney and ovary. In humans, the gene encoding for the netrin-4 protein is localized to chromosome 12q22-q23. High levels of netrin-4 mRNA have also been detected in many cells and tissues, including cerebral cortex, hippocampus, amygdaloid nuclei and Purkinje cells. Netrin-4 is important in neural, kidney and vascular development. |
| Application Dilution | |
| WB | IFIHC-P1:50-200 |
| IF | IHC-F1:50-200 |
Frequently asked questions
Which applications is Netrin 4 Polyclonal Antibody, Cy3 Conjugated (bs-10065R-Cy3) validated for, and at what dilution?
bs-10065R-Cy3 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-10065R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Netrin 4 Polyclonal Antibody, Cy3 Conjugated (bs-10065R-Cy3)?
KLH conjugated synthetic peptide derived from human Netrin 4
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-10065R-Cy3)?
Cy3 on bs-10065R-Cy3: excitation / emission 512,550nm/570,615nm.