PDGF-A Polyclonal Antibody, PE-Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-10073R-PE-Cy5.5-100uL |
| Product Name | PDGF-A Polyclonal Antibody, PE-Cy5.5 Conjugated |
| Applications |
WB
FCM
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat, Rabbit |
| Specifications | |
| Conjugation | PE-Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from rabbit PDGF-A |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5154 |
| Swiss Prot | P04085 |
| Subcellular location | Secreted |
| Synonyms | PDGFA; PDGF AA; PDGF-AA; PDGF A chain; PDGF subunit A; PDGF-1; PDGF1; PDGFA; PDGFA_HUMAN; Platelet derived growth factor A chain precursor; Platelet derived growth factor alpha polypeptide; Platelet-derived growth factor A chain; Platelet-derived growth factor alpha polypeptide; Platelet-derived growth factor subunit A. |
| Background | Platelet-derived growth factor is a potent mitogen for cells of mesenchymal origin. Binding of this growth factor to its affinity receptor elicits a variety of cellular responses. It is released by platelets upon wounding and plays an important role in stimulating adjacent cells to grow and thereby heal the wound. Antiparallel disulfide-linked dimer of nonidentical (A and B) chains. Homodimers of A and B chains are implicated in transformation processes. The long form contains a basic insert which acts as a cell retention signal. A-A and B-B, as well as A-B, dimers can bind to the PDGF receptor. It belongs to the PDGF/VEGF growth factor family. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is PDGF-A Polyclonal Antibody, PE-Cy5.5 Conjugated (BS-10073R-PE-CY5-5) validated for, and at what dilution?
BS-10073R-PE-CY5-5 is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PDGF-A Polyclonal Antibody, PE-Cy5.5 Conjugated (BS-10073R-PE-CY5-5) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Rabbit.
How should BS-10073R-PE-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PDGF-A Polyclonal Antibody, PE-Cy5.5 Conjugated (BS-10073R-PE-CY5-5)?
KLH conjugated synthetic peptide derived from rabbit PDGF-A
What are the excitation and emission wavelengths of the PE-Cy5.5 conjugate (BS-10073R-PE-CY5-5)?
PE-Cy5.5 on BS-10073R-PE-CY5-5: excitation / emission 488nm/694nm.