Nidogen2 Polyclonal Antibody, AbBy Fluor-647 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-10075R-BF647-100uL |
| Product Name | Nidogen2 Polyclonal Antibody, AbBy Fluor-647 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Pig, Horse |
| Specifications | |
| Conjugation | AbBy Fluor-647 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Nidogen2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 22795 |
| Swiss Prot | Q14112 |
| Subcellular location | Secreted, Cell membrane, Extracellular matrix |
| Synonyms | NID 2; NID2; Nidogen2; Nidogen 2; Nidogen-2; Osteonidogen; NID2_HUMAN. |
| Background | Nidogen 2 is an adhesion glycoprotein which is widely distributed in basement membranes. Binds to collagens I and IV, to perlecan and to laminin 1. Does not bind fibulins. It probably has a role in cell extracellular matrix interactions. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Nidogen2 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-10075R-BF647) validated for, and at what dilution?
bs-10075R-BF647 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-10075R-BF647 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Nidogen2 Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-10075R-BF647)?
KLH conjugated synthetic peptide derived from human Nidogen2
What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bs-10075R-BF647)?
AbBy Fluor-647 on bs-10075R-BF647: excitation / emission 650nm/665nm.