PLAG1 Polyclonal Antibody

$Lane 1: mouse lung lysates probed with \\tPLAG1 Polyclonal Antibody, Unconjugated (bs-10092R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:10000 for 90 min at 37˚C.$

Lane 1: mouse lung lysates probed with \\tPLAG1 Polyclonal Antibody, Unconjugated (bs-10092R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:10000 for 90 min at 37˚C.

Applications

WB
IHC-P
IHC-F
IF

Reactivity

Mouse

Predicted Reactivity

Human
Rat
Dog
Cow
Pig
Horse
Rabbit

Overview
Catalog #	bs-10092R
Product Name	PLAG1 Polyclonal Antibody
Applications	WB, IHC-P, IHC-F, IF
Reactivity	Mouse
Predicted Reactivity	Human, Rat, Dog, Cow, Pig, Horse, Rabbit
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human PLAG1
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	5324
Swiss Prot	Q6DJT9
Subcellular location	Nucleus
Synonyms	PSA; SGPA; ZNF912; Zinc finger protein PLAG1; Pleiomorphic adenoma gene 1 protein; PLAG1
Background	Transcription factor whose activation results in up-regulation of target genes, such as IGFII, leading to uncontrolled cell proliferation: when overexpressed in cultured cells, higher proliferation rate and transformation are observed. Other target genes such as CRLF1, CRABP2, CRIP2, PIGF are strongly induced in cells with PLAG1 induction. Proto-oncogene whose ectopic expression can trigger the development of pleomorphic adenomas of the salivary gland and lipoblastomas. Overexpression is associated with up-regulation of IGFII, is frequently observed in hepatoblastoma, common primary liver tumor in childhood. Cooperates with CBFB-MYH11, a fusion gene important for myeloid leukemia.
Application Dilution
WB	1:300-5000
IHC-P	ELISA1:500-1000
IHC-F
IF