phospho-Ron (Tyr1238+Tyr1239) Polyclonal Antibody, PE-Cy5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-10118R-PE-Cy5 |
| Product Name | phospho-Ron (Tyr1238+Tyr1239) Polyclonal Antibody, PE-Cy5 Conjugated |
| Applications |
WB
FCM
|
| Reactivity | Human, Rat |
| Predicted Reactivity | Mouse |
| Specifications | |
| Conjugation | PE-Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human MST1R around the phosphorylation site of Tyr1238+Tyr1239 |
| Modification Site | Tyr1238+Tyr1239 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 4486 |
| Swiss Prot | Q04912 |
| Subcellular location | Cell membrane |
| Synonyms | RON; PTK8; CD136; CDw136; Macrophage-stimulating protein receptor; MSP receptor; Protein-tyrosine kinase 8; p185-Ron; MST1R |
| Background | Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to MST1 ligand. Regulates many physiological processes including cell survival, migration and differentiation. Ligand binding at the cell surface induces autophosphorylation of RON on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1 or the adapter GAB1. Recruitment of these downstream effectors by RON leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. RON signaling activates the wound healing response by promoting epithelial cell migration, proliferation as well as survival at the wound site. Plays also a role in the innate immune response by regulating the migration and phagocytic activity of macrophages. Alternatively, RON can also promote signals such as cell migration and proliferation in response to growth factors other than MST1 ligand. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
Frequently asked questions
Which applications is phospho-Ron (Tyr1238+Tyr1239) Polyclonal Antibody, PE-Cy5 Conjugated (bs-10118R-PE-Cy5) validated for, and at what dilution?
bs-10118R-PE-Cy5 is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-Ron (Tyr1238+Tyr1239) Polyclonal Antibody, PE-Cy5 Conjugated (bs-10118R-PE-Cy5) react with?
Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse.
How should bs-10118R-PE-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-Ron (Tyr1238+Tyr1239) Polyclonal Antibody, PE-Cy5 Conjugated (bs-10118R-PE-Cy5)?
KLH conjugated synthetic phosphopeptide derived from human MST1R around the phosphorylation site of Tyr1238+Tyr1239
What are the excitation and emission wavelengths of the PE-Cy5 conjugate (bs-10118R-PE-Cy5)?
PE-Cy5 on bs-10118R-PE-Cy5: excitation / emission 488nm/670nm.