phospho-CD130/gp130 (Ser782) Polyclonal Antibody, Cy5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-10122R-Cy5-100uL |
| Product Name | phospho-CD130/gp130 (Ser782) Polyclonal Antibody, Cy5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human, Rat, Rabbit |
| Predicted Reactivity | Mouse, Dog, Cow, Pig, Horse, Chicken |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human gp130 around the phosphorylation site of Ser782 |
| Modification Site | Ser782 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3572 |
| Swiss Prot | P40189 |
| Subcellular location | Secreted, Cell membrane |
| Synonyms | gp130 phospho S782; gp130 phospho Ser782; p-gp130 Ser782; Interleukin-6 receptor subunit beta; IL-6R-beta; Interleukin-6 signal transducer; Membrane glycoprotein 130; gp130; CDw130; Oncostatin-M receptor subunit alpha; CD_antigen; CD130; GP130 RAPS; gp130 transducer chain; GP130-RAPS; IL6 ST; IL6R-beta; IL6ST.IL6R-beta; IL6RB_HUMAN; IL-6 receptor subunit beta; IL-6R subunit beta; IL-6RB. |
| Background | CD130 is a signal transducer shared by many cytokines, including interleukin 6 (IL6), ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF), and oncostatin M (OSM). This protein functions as a part of the cytokine receptor complex. The activation of this protein is dependent upon the binding of cytokines to their receptors. vIL6, a protein related to IL6 and encoded by the Kaposi sarcoma-associated herpesvirus, can bypass the interleukin 6 receptor (IL6R) and directly activate this protein. Knockout studies in mice suggested a critical role of the gene encoding this protein in regulating myocyte apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been described. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-CD130/gp130 (Ser782) Polyclonal Antibody, Cy5 Conjugated (bs-10122R-Cy5) validated for, and at what dilution?
bs-10122R-Cy5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-CD130/gp130 (Ser782) Polyclonal Antibody, Cy5 Conjugated (bs-10122R-Cy5) react with?
Tested reactivity: Human, Rat, Rabbit. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Dog, Cow, Pig, Horse, Chicken.
How should bs-10122R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-CD130/gp130 (Ser782) Polyclonal Antibody, Cy5 Conjugated (bs-10122R-Cy5)?
KLH conjugated synthetic phosphopeptide derived from human gp130 around the phosphorylation site of Ser782
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-10122R-Cy5)?
Cy5 on bs-10122R-Cy5: excitation / emission 625,650nm/670nm.