Phospho-LAT (Tyr161) Polyclonal Antibody, RBITC Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-10127R-RBITC |
| Product Name | Phospho-LAT (Tyr161) Polyclonal Antibody, RBITC Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Predicted Reactivity | Human, Mouse, Rat, Cow |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human LAT around the phosphorylation site of Tyr161 |
| Modification Site | Tyr161 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 27040 |
| Swiss Prot | O43561 |
| Subcellular location | Cell membrane |
| Synonyms | LAT1; pp36; Linker for activation of T-cells family member 1; 36 kDa phospho-tyrosine adapter protein; p36-38; LAT |
| Background | Required for TCR (T-cell antigen receptor)- and pre-TCR-mediated signaling, both in mature T-cells and during their development. Involved in FCGR3 (low affinity immunoglobulin gamma Fc region receptor III)-mediated signaling in natural killer cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Couples activation of these receptors and their associated kinases with distal intracellular events such as mobilization of intracellular calcium stores, PKC activation, MAPK activation or cytoskeletal reorganization through the recruitment of PLCG1, GRB2, GRAP2, and other signaling molecules. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Phospho-LAT (Tyr161) Polyclonal Antibody, RBITC Conjugated (bs-10127R-RBITC) validated for, and at what dilution?
bs-10127R-RBITC is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bs-10127R-RBITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-LAT (Tyr161) Polyclonal Antibody, RBITC Conjugated (bs-10127R-RBITC)?
KLH conjugated synthetic phosphopeptide derived from human LAT around the phosphorylation site of Tyr161
What are the excitation and emission wavelengths of the RBITC conjugate (bs-10127R-RBITC)?
RBITC on bs-10127R-RBITC: excitation / emission 540nm/625nm.