Phospho-LAT (Tyr200) Polyclonal Antibody, AbBy Fluor-405 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-10128R-BF405 |
| Product Name | Phospho-LAT (Tyr200) Polyclonal Antibody, AbBy Fluor-405 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Cow, Horse, Rabbit |
| Specifications | |
| Conjugation | AbBy Fluor-405 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human LAT around the phosphorylation site of Tyr200 |
| Modification Site | Tyr200 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 27040 |
| Subcellular location | Cell membrane |
| Synonyms | Linker for activation of T cell 1; 36 kDa phospho tyrosine adapter protein; LAT 1; LAT1; Linker for activation of T cells; Linker for activation of T cells family member 1; p36 38; pp36; LAT_HUMAN. |
| Background | Linker for activation of T cells (LAT) is an integral membrane protein (36 to 38 kD), which plays an important role in linking engagement of the TCR to the biochemical events of T cell activation. LAT antibody stains thymus and peripheral lymphoid tissues such as T cell areas in lymph nodes and spleen. LAT is expressed in T lymphocytes in interstitial spaces, platelets and megakaryocytes. LAT is not expressed in B cells, macrophages, plasma cells, monocytes, epithelial histiocytes and dendritic cells. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Phospho-LAT (Tyr200) Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-10128R-BF405) validated for, and at what dilution?
bs-10128R-BF405 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-LAT (Tyr200) Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-10128R-BF405) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Horse, Rabbit.
How should bs-10128R-BF405 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-LAT (Tyr200) Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-10128R-BF405)?
KLH conjugated synthetic phosphopeptide derived from human LAT around the phosphorylation site of Tyr200
What are the excitation and emission wavelengths of the AbBy Fluor-405 conjugate (bs-10128R-BF405)?
AbBy Fluor-405 on bs-10128R-BF405: excitation / emission 401nm/421nm.