phospho-Eph receptor B1 (Tyr928) Polyclonal Antibody

$Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti phospho-EphB1\/Eph receptor B1(Tyr928) Antibody, Unconjugated (bs-10138R) at 1:200 followed by conjugation to the secondary antibody and DAB staining$

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti phospho-EphB1\/Eph receptor B1(Tyr928) Antibody, Unconjugated (bs-10138R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Mouse ovary lysates probed with Eph receptor B1 (Tyr928) Polyclonal Antibody, Unconjugated (bs-10138R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Lane 1: Mouse Testis tissue lysates; Lane 2: Mouse NIH\/3T3 cell lysates; Lane 3: Human U251 cell lysates; Lane 4: Human U87MG cell lysates; Lane 5: Human MCF-7 cell lysates; Lane 6: Human U-2 OS cell lysates probed with phospho-Eph receptor B1 (Tyr928) Polyclonal Antibody, Unconjugated (bs-10138R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Testis tissue lysates; Lane 2: Mouse NIH\/3T3 cell lysates; Lane 3: Human U251 cell lysates; Lane 4: Human U87MG cell lysates; Lane 5: Human MCF-7 cell lysates; Lane 6: Human U-2 OS cell lysates probed with phospho-Eph receptor B1 (Tyr928) Polyclonal Antibody, Unconjugated (bs-10138R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-10138R
Product Name	phospho-Eph receptor B1 (Tyr928) Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic phosphopeptide derived from human EphB1 around the phosphorylation site of Tyr928
Modification Site	Tyr928
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	2047
Swiss Prot	P54762
Subcellular location	Cytoplasm, Cell membrane
Synonyms	ELK; NET; Hek6; EPHT2; Ephrin type-B receptor 1; EPH tyrosine kinase 2; EPH-like kinase 6; EK6; Neuronally-expressed EPH-related tyrosine kinase; Tyrosine-protein kinase receptor EPH-2; EPHB1
Background	Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Cognate/functional ephrin ligands for this receptor include EFNB1, EFNB2 and EFNB3. During nervous system development, regulates retinal axon guidance redirecting ipsilaterally ventrotemporal retinal ganglion cells axons at the optic chiasm midline. This probably requires repulsive interaction with EFNB2. In the adult nervous system together with EFNB3, regulates chemotaxis, proliferation and polarity of the hippocampus neural progenitors. In addition to its role in axon guidance plays also an important redundant role with other ephrin-B receptors in development and maturation of dendritic spines and synapse formation. May also regulate angiogenesis. More generally, may play a role in targeted cell migration and adhesion. Upon activation by EFNB1 and probably other ephrin-B ligands activates the MAPK/ERK and the JNK signaling cascades to regulate cell migration and adhesion respectively.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200