phospho-Eph receptor B1 (Tyr968) Polyclonal Antibody, AbBy Fluor-405 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-10139R-BF405 |
| Product Name | phospho-Eph receptor B1 (Tyr968) Polyclonal Antibody, AbBy Fluor-405 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human |
| Specifications | |
| Conjugation | AbBy Fluor-405 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human EphB1 around the phosphorylation site of Tyr968 |
| Modification Site | Tyr968 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 2047 |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | Eph receptor B1phospho Y968; EphB1phospho Y968Cek 6; Elkh; Eph tyrosine kinase 2; EPH2; EphB1; Ephrin receptor Eph B1 precursor; Ephrin type B receptor 1; HEK 6; NET; Neuronally expressed EPH related tyrosine kinase; Tyrosine protein kinase receptor EPH 2. |
| Background | EphB1, previously known as Elk (eph like kinase), is a receptor tyrosine kinase of the highly tissue restricted family of eph proteins. EphB1 and other ephB family members are type 1 membrane spanning proteins, comprised of immunoglobulin, fibronectin type III, and cysteine rich subdomains in the ecto domain, and the single uninterrupted cytoplasmic tyrosine kinase domain upstream of a carboxyterminal sterile alpha motif (SAM) domain. EphB family proteins bind ephrins of the B class. EphB1 is expressed predominately in developing neural structures in embryos, and in vascular epithelium of kidney, and other tissues. Upon binding to alternatively oligomerized ephrin B1, EphB1 signals regulation of cell attachment and cell to cell assembly. Members of this protein family are implicated in neuronal and vascular cell targeting. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-Eph receptor B1 (Tyr968) Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-10139R-BF405) validated for, and at what dilution?
bs-10139R-BF405 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-Eph receptor B1 (Tyr968) Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-10139R-BF405) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human.
How should bs-10139R-BF405 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-Eph receptor B1 (Tyr968) Polyclonal Antibody, AbBy Fluor-405 Conjugated (bs-10139R-BF405)?
KLH conjugated synthetic phosphopeptide derived from human EphB1 around the phosphorylation site of Tyr968
What are the excitation and emission wavelengths of the AbBy Fluor-405 conjugate (bs-10139R-BF405)?
AbBy Fluor-405 on bs-10139R-BF405: excitation / emission 401nm/421nm.