SCNN1D Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-10141R-Cy3 |
| Product Name | SCNN1D Polyclonal Antibody, Cy3 Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Rat |
| Predicted Reactivity | Human |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human SCNN1D |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 6339 |
| Swiss Prot | P51172 |
| Subcellular location | Cell membrane |
| Synonyms | ENaCd; SCNED; dNaCh; ENaCdelta; Amiloride-sensitive sodium channel subunit delta; Delta-NaCH; Epithelial Na(+) channel subunit delta; Delta-ENaC; Nonvoltage-gated sodium channel 1 subunit delta; SCNN1D |
| Background | Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is SCNN1D Polyclonal Antibody, Cy3 Conjugated (bs-10141R-Cy3) validated for, and at what dilution?
bs-10141R-Cy3 is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does SCNN1D Polyclonal Antibody, Cy3 Conjugated (bs-10141R-Cy3) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human.
How should bs-10141R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise SCNN1D Polyclonal Antibody, Cy3 Conjugated (bs-10141R-Cy3)?
KLH conjugated synthetic peptide derived from human SCNN1D
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-10141R-Cy3)?
Cy3 on bs-10141R-Cy3: excitation / emission 512,550nm/570,615nm.