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CD35 Polyclonal Antibody, RBITC Conjugated

Applications

  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bs-10144R-RBITC-100uL
Product Name CD35 Polyclonal Antibody, RBITC Conjugated
Applications
IF(IHC-P) IF(IHC-F) IF(ICC)
Specificity May recognize Complement component receptor 1-like protein in certain tissues, which is expressed in fetal liver and to a lesser extent in fetal spleen and thymus.
Reactivity Human, Mouse, Rat
Specifications
Conjugation RBITC
Host Rabbit
Source KLH conjugated synthetic peptide derived from human CR1/CD35
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 1378
Swiss Prot P17927
Subcellular location Cell membrane
Synonyms KN; C3BR; C4BR; CD35; Complement receptor type 1; C3b/C4b receptor; CR1
Background Mediates cellular binding of particles and immune complexes that have activated complement.
Application Dilution
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is CD35 Polyclonal Antibody, RBITC Conjugated (bs-10144R-RBITC) validated for, and at what dilution?

bs-10144R-RBITC is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does CD35 Polyclonal Antibody, RBITC Conjugated (bs-10144R-RBITC) react with?

Tested reactivity: Human, Mouse, Rat.

How should bs-10144R-RBITC be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise CD35 Polyclonal Antibody, RBITC Conjugated (bs-10144R-RBITC)?

KLH conjugated synthetic peptide derived from human CR1/CD35

What are the excitation and emission wavelengths of the RBITC conjugate (bs-10144R-RBITC)?

RBITC on bs-10144R-RBITC: excitation / emission 540nm/625nm.

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