Phospho-ELk1 (Ser383) Polyclonal Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-10154R-100uL |
| Product Name | Phospho-ELk1 (Ser383) Polyclonal Antibody |
| Applications |
WB
ELISA
IHC-P
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat, Dog, Cow, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human ELK1 around the phosphorylation site of Ser383 |
| Modification Site | Ser383 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 2002 |
| Swiss Prot | P19419 |
| Subcellular location | Nucleus |
| Synonyms | ETS domain-containing protein Elk-1; ELK1 |
| Background | Transcription factor that binds to purine-rich DNA sequences. Forms a ternary complex with SRF and the ETS and SRF motifs of the serum response element (SRE) on the promoter region of immediate early genes such as FOS and IER2. Induces target gene transcription upon JNK-signaling pathway stimulation (By similarity). |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
| IHC-P | 1:200-400 |
Frequently asked questions
Which applications is Phospho-ELk1 (Ser383) Polyclonal Antibody (bs-10154R) validated for, and at what dilution?
bs-10154R is validated for WB (1:300-5000); ELISA (1:500-1000); IHC-P (1:200-400). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-ELk1 (Ser383) Polyclonal Antibody (bs-10154R) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Dog, Cow, Pig, Horse, Rabbit.
How should bs-10154R be stored?
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-ELk1 (Ser383) Polyclonal Antibody (bs-10154R)?
KLH conjugated synthetic phosphopeptide derived from human ELK1 around the phosphorylation site of Ser383