ARHGEF11 Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-10157R-Cy5.5-100uL |
| Product Name | ARHGEF11 Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human PDZ RHOGEF/GTRAP48 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Cytoplasm, Cell membrane |
| Synonyms | PDZ-RhoGEF; ARHGB_HUMAN; ARHGEF 11; ARHGEF11; DKFZp667F1223; Glutamate transporter EAAT4 associated protein 48; GTRAP 48; GTRAP48; PDZ RhoGEF; Rho guanine exchange factor GEF 11; Rho guanine nucleotide exchange factor GEF 11; Rho guanine nucleotide exchange factor 11; RhoA specic guanine nucleotide exchange factor; RhoGEF glutamate transport modulator. |
| Background | Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form a complex with G proteins and stimulate Rho-dependent signals. A similar protein in rat interacts with glutamate transporter EAAT4 and modulates its glutamate transport activity. Expression of the rat protein induces the reorganization of the actin cytoskeleton and its overexpression induces the formation of membrane ruffling and filopodia. Two alternative transcripts encoding different isoforms have been described. [provided by RefSeq, Jul 2008]. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is ARHGEF11 Polyclonal Antibody, Cy5.5 Conjugated (BS-10157R-CY5-5) validated for, and at what dilution?
BS-10157R-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ARHGEF11 Polyclonal Antibody, Cy5.5 Conjugated (BS-10157R-CY5-5) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Pig, Horse, Rabbit.
How should BS-10157R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ARHGEF11 Polyclonal Antibody, Cy5.5 Conjugated (BS-10157R-CY5-5)?
KLH conjugated synthetic peptide derived from human PDZ RHOGEF/GTRAP48
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-10157R-CY5-5)?
Cy5.5 on BS-10157R-CY5-5: excitation / emission 675nm/694nm.