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CYP11B2 Polyclonal Antibody, PerCP Conjugated

Applications

  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Rabbit
Overview
Catalog # bs-10161R-PerCP-100uL
Product Name CYP11B2 Polyclonal Antibody, PerCP Conjugated
Applications
IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Mouse, Rat
Predicted Reactivity Rabbit
Specifications
Conjugation PerCP
Host Rabbit
Source KLH conjugated synthetic peptide derived from human CYP11B2
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Subcellular location Cytoplasm
Synonyms CYP 11B2; CYP11B2; CYPXI11B2; Cytochrome P450 1111B2; Cytochrome P450 1111B2 mitochondrial; Cytochrome P450 family 11 subfamily B polypeptide 2; Cytochrome P450 subfamily XIB cholesterol side chain cleavage; Cytochrome P450 subfamily XI11B2; Cytochrome P450C1111B2; C11B2_HUMAN.
Background This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the mitochondrial inner membrane. The enzyme has steroid 18-hydroxylase activity to synthesize aldosterone and 18-oxocortisol as well as steroid 11 beta-hydroxylase activity. Mutations in this gene cause corticosterone methyl oxidase deficiency. [provided by RefSeq, Jul 2008].
Application Dilution
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is CYP11B2 Polyclonal Antibody, PerCP Conjugated (bs-10161R-PerCP) validated for, and at what dilution?

bs-10161R-PerCP is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does CYP11B2 Polyclonal Antibody, PerCP Conjugated (bs-10161R-PerCP) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Rabbit.

How should bs-10161R-PerCP be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise CYP11B2 Polyclonal Antibody, PerCP Conjugated (bs-10161R-PerCP)?

KLH conjugated synthetic peptide derived from human CYP11B2

What are the excitation and emission wavelengths of the PerCP conjugate (bs-10161R-PerCP)?

PerCP on bs-10161R-PerCP: excitation / emission 482nm/677nm.