Spectrin (alpha + beta) Polyclonal Antibody
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-10190R-100uL |
| Product Name | Spectrin (alpha + beta) Polyclonal Antibody |
| Applications |
WB
ELISA
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | Unconjugated |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Spectrin alpha chain, erythrocytic 1 |
| Immunogen Range | 2151-2350/2419 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| Target | |
| Gene ID | 6708 |
| Subcellular location | Cytoplasm |
| Synonyms | Alpha I spectrin; EL 2; EL2; Elliptocytosis 2; Elliptocytosis2; Erythrocyte alpha spectrin; erythrocyte; Erythroid alpha spectrin; Erythroid alpha-spectrin; Erythroid spectrin alpha; HPP; HS3; Spectrin alpha chain; Spectrin alpha chain erythrocyte; Spectrin alpha erythrocytic 1; SPH3; SPTA 1; SPTA; SPTA1; SPTA1_HUMAN; Spectrin alpha chain, erythrocytic 1. |
| Background | Spectrin is an actin crosslinking and molecular scaffold protein that links the plasma membrane to the actin cytoskeleton, and functions in the determination of cell shape, arrangement of transmembrane proteins, and organization of organelles. It is a tetramer made up of alpha-beta dimers linked in a head-to-head arrangement. This gene is one member of a family of alpha-spectrin genes. The encoded protein is primarily composed of 22 spectrin repeats which are involved in dimer formation. It forms weaker tetramer interactions than non-erythrocytic alpha spectrin, which may increase the plasma membrane elasticity and deformability of red blood cells. Mutations in this gene result in a variety of hereditary red blood cell disorders, including elliptocytosis type 2, pyropoikilocytosis, and spherocytic hemolytic anemia. [provided by RefSeq, Jul 2008]. |
| Application Dilution | |
| WB | 1:300-5000 |
| ELISA | 1:500-1000 |
Frequently asked questions
Which applications is Spectrin (alpha + beta) Polyclonal Antibody (bs-10190R) validated for, and at what dilution?
bs-10190R is validated for WB (1:300-5000); ELISA (1:500-1000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Spectrin (alpha + beta) Polyclonal Antibody (bs-10190R) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Pig, Horse, Rabbit.
How should bs-10190R be stored?
Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Spectrin (alpha + beta) Polyclonal Antibody (bs-10190R)?
KLH conjugated synthetic peptide derived from human Spectrin alpha chain, erythrocytic 1 Immunogen range: 2151-2350/2419.