HPV16 E7 Polyclonal Antibody, APC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-10202R-APC |
| Product Name | HPV16 E7 Polyclonal Antibody, APC Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Virus |
| Specifications | |
| Conjugation | APC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from HPV16 E7 protein |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1489079 |
| Synonyms | E7; Human Papilloma Virus; Human papillomavirus type 16 E7; Human papillomavirus type 16 E7; Protein E7; Human Papillomavirus 16 E7; HPV16 E7; E7 protein HPV16; VE7_HPV16. |
| Background | Human papilloma viruses (HPVs) can be classified as either high risk or low risk according to their association with cancer. HPV16 and HPV18 are the most common of the high risk group while HPV6 and HPV11 are among the low risk types. Approximately 90% of cervical cancers contain HPV DNA of the high risk types. Mutational analysis have shown that the E6 and E7 genes of the high risk HPVs are necessary and sufficient for HPV transforming function. The specific interactions of the E6 and E7 proteins with p53 and pRB, respectively, correlate with HPV high and low risk classifications. The high risk HPV E7 proteins bind to pRB with a higher affinity than do the low risk HPV proteins, and only the high risk HPV E6 proteins form detectable complexes with p53 in vitro. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is HPV16 E7 Polyclonal Antibody, APC Conjugated (bs-10202R-APC) validated for, and at what dilution?
bs-10202R-APC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does HPV16 E7 Polyclonal Antibody, APC Conjugated (bs-10202R-APC) react with?
Tested reactivity: Virus.
How should bs-10202R-APC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise HPV16 E7 Polyclonal Antibody, APC Conjugated (bs-10202R-APC)?
KLH conjugated synthetic peptide derived from HPV16 E7 protein
What are the excitation and emission wavelengths of the APC conjugate (bs-10202R-APC)?
APC on bs-10202R-APC: excitation / emission 650nm/660nm.