FoxP3 Polyclonal Antibody, PE Conjugated

$Mouse spleen cells were fixed with 4% PFA for 10min at room temperature\uff0cpermeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with FoxP3 Polyclonal Antibody, PE Conjugated Antibody\uff08bs-10211R-FITC\uff09 at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange).$

Mouse spleen cells were fixed with 4% PFA for 10min at room temperature\uff0cpermeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with FoxP3 Polyclonal Antibody, PE Conjugated Antibody\uff08bs-10211R-FITC\uff09 at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange).

$Mouse spleen cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103. Cells were then stained with FoxP3 Polyclonal Antibody(bs-10211R-PE)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).$

Mouse spleen cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103. Cells were then stained with FoxP3 Polyclonal Antibody(bs-10211R-PE)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Applications

Reactivity

Human
Mouse
Rat

Predicted Reactivity

Dog
Cow
Sheep
Pig
Horse
Rabbit
Guinea Pig

Overview
Catalog #	bs-10211R-PE
Product Name	FoxP3 Polyclonal Antibody, PE Conjugated
Applications	WB, FCM
Reactivity	Human, Mouse, Rat
Predicted Reactivity	Dog, Cow, Sheep, Pig, Horse, Rabbit, Guinea Pig
Specifications
Conjugation	PE
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human FoxP3
Immunogen Range	331-431/431
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Condition	Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Subcellular location	Nucleus
Synonyms	AIID; AIID; DIETER; DIETER; Forkhead box P3; Forkhead box protein P3; foxp3; foxp3; FOXP3_HUMAN; FOXP3delta7; Immune dysregulation polyendocrinopathy enteropathy X linked; Immunodeficiency polyendocrinopathy enteropathy X linked; IPEX; IPEX; JM2; JM2; MGC141961; MGC141963; OTTHUMP00000025832; OTTHUMP00000025833; OTTHUMP00000226737; PIDX; PIDX; SCURFIN; SCURFIN; XPID; XPID.
Background	The protein encoded by this gene is a member of the forkhead/winged-helix family of transcriptional regulators. Defects in this gene are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX), also known as X-linked autoimmunity-immunodeficiency syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008].
Application Dilution
WB	1:300-5000
FCM	1:20-100

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