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FoxP3 Polyclonal Antibody, PE Conjugated

Mouse spleen cells were fixed with 4% PFA for 10min at room temperature\uff0cpermeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with FoxP3 Polyclonal Antibody, PE Conjugated Antibody\uff08bs-10211R-FITC\uff09 at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange).
Mouse spleen cells were fixed with 4% PFA for 10min at room temperature\uff0cpermeabilized with 90% ice-cold methanol for 20 min at -20\u2103, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with FoxP3 Polyclonal Antibody, PE Conjugated Antibody\uff08bs-10211R-FITC\uff09 at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green) and isotype control (orange).
Mouse spleen cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103. Cells were then stained with FoxP3 Polyclonal Antibody(bs-10211R-PE)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Mouse spleen cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20\u2103. Cells were then stained with FoxP3 Polyclonal Antibody(bs-10211R-PE)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).
Bioss conjugated antibody and packaging; contents and labels vary by product.
Bioss conjugated antibody and packaging; contents and labels vary by product.

Applications

  • WB
  • FCM
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Dog
  • Cow
  • Sheep
  • Pig
  • Horse
  • Rabbit
  • GuineaPig
Overview
Catalog # bs-10211R-PE
Product Name FoxP3 Polyclonal Antibody, PE Conjugated
Applications
WB FCM IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Mouse, Rat
Predicted Reactivity Dog, Cow, Sheep, Pig, Horse, Rabbit, GuineaPig
Specifications
Conjugation PE
Host Rabbit
Source KLH conjugated synthetic peptide derived from human FoxP3
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Subcellular location Nucleus
Synonyms AIID; AIID; DIETER; DIETER; Forkhead box P3; Forkhead box protein P3; foxp3; foxp3; FOXP3_HUMAN; FOXP3delta7; Immune dysregulation polyendocrinopathy enteropathy X linked; Immunodeficiency polyendocrinopathy enteropathy X linked; IPEX; IPEX; JM2; JM2; MGC141961; MGC141963; OTTHUMP00000025832; OTTHUMP00000025833; OTTHUMP00000226737; PIDX; PIDX; SCURFIN; SCURFIN; XPID; XPID.
Background The protein encoded by this gene is a member of the forkhead/winged-helix family of transcriptional regulators. Defects in this gene are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX), also known as X-linked autoimmunity-immunodeficiency syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008].
Application Dilution
WB 1:300-5000
FCM 1:20-100
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Frequently asked questions

Which applications is FoxP3 Polyclonal Antibody, PE Conjugated (bs-10211R-PE) validated for, and at what dilution?

bs-10211R-PE is validated for WB (1:300-5000); FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does FoxP3 Polyclonal Antibody, PE Conjugated (bs-10211R-PE) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Sheep, Pig, Horse, Rabbit, GuineaPig.

How should bs-10211R-PE be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise FoxP3 Polyclonal Antibody, PE Conjugated (bs-10211R-PE)?

KLH conjugated synthetic peptide derived from human FoxP3

What are the excitation and emission wavelengths of the PE conjugate (bs-10211R-PE)?

PE on bs-10211R-PE: excitation / emission 496,564nm/578nm.

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