CHI3L1 Polyclonal Antibody, PerCP Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-10215R-PerCP-100uL |
| Product Name | CHI3L1 Polyclonal Antibody, PerCP Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat |
| Specifications | |
| Conjugation | PerCP |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from Human CHI3L1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1116 |
| Swiss Prot | P36222 |
| Subcellular location | Cytoplasm, Secreted |
| Synonyms | GP39; ASRT7; GP-39; YKL4; CGP-39; YKL-4; YYL-4; HC-gp39; HCGP-3P; hCGP-39; Chitinase-3-like protein 1; 39 kDa synovial protein; Cartilage glycoprotein 39; CHI3L1 |
| Background | Carbohydrate-binding lectin with a preference for chitin. Has no chitinase activity. May play a role in tissue remodeling and in the capacity of cells to respond to and cope with changes in their environment. Plays a role in T-helper cell type 2 (Th2) inflammatory response and IL-13-induced inflammation, regulating allergen sensitization, inflammatory cell apoptosis, dendritic cell accumulation and M2 macrophage differentiation. Facilitates invasion of pathogenic enteric bacteria into colonic mucosa and lymphoid organs. Mediates activation of AKT1 signaling pathway and subsequent IL8 production in colonic epithelial cells. Regulates antibacterial responses in lung by contributing to macrophage bacterial killing, controlling bacterial dissemination and augmenting host tolerance. Also regulates hyperoxia-induced injury, inflammation and epithelial apoptosis in lung. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is CHI3L1 Polyclonal Antibody, PerCP Conjugated (bs-10215R-PerCP) validated for, and at what dilution?
bs-10215R-PerCP is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CHI3L1 Polyclonal Antibody, PerCP Conjugated (bs-10215R-PerCP) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat.
How should bs-10215R-PerCP be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CHI3L1 Polyclonal Antibody, PerCP Conjugated (bs-10215R-PerCP)?
KLH conjugated synthetic peptide derived from Human CHI3L1
What are the excitation and emission wavelengths of the PerCP conjugate (bs-10215R-PerCP)?
PerCP on bs-10215R-PerCP: excitation / emission 482nm/677nm.