Nanos3 Polyclonal Antibody, AbBy Fluor-750 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-10227R-BF750-100uL |
| Product Name | Nanos3 Polyclonal Antibody, AbBy Fluor-750 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Cow, Sheep, Pig |
| Specifications | |
| Conjugation | AbBy Fluor-750 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Nanos3 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 342977 |
| Swiss Prot | P60323 |
| Subcellular location | Secreted, Cell membrane, Extracellular matrix |
| Synonyms | NOS3; NANOS1L; Nanos homolog 3; NOS-3; NANOS3 |
| Background | Plays a role in the maintenance of the undifferentiated state of germ cells regulating the spermatogonia cell cycle and inducing a prolonged transit in G1 phase. Affects cell proliferation probably by repressing translation of specific mRNAs. Maintains the germ cell lineage by suppressing both Bax-dependent and -independent apoptotic pathways. Essential in the early stage embryo to protect the migrating primordial germ cells (PGCs) from apoptosis. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is Nanos3 Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-10227R-BF750) validated for, and at what dilution?
bs-10227R-BF750 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Nanos3 Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-10227R-BF750) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Cow, Sheep, Pig.
How should bs-10227R-BF750 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Nanos3 Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-10227R-BF750)?
KLH conjugated synthetic peptide derived from human Nanos3
What are the excitation and emission wavelengths of the AbBy Fluor-750 conjugate (bs-10227R-BF750)?
AbBy Fluor-750 on bs-10227R-BF750: excitation / emission 749nm/775nm.