Inhibin Alpha Polyclonal Antibody

Formalin-fixed and paraffin embedded human placenta tissue labeled with Rabbit Anti-Inhibin Alpha Polyclonal Antibody, Unconjugated (bs-10237R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Mouse intestinal lysates probed with Rabbit Anti-Inhibin Alpha Polyclonal Antibody, Unconjugated (bs-10237R) at 1:300 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:5000 for 90min at 37˚C.

$Paraformaldehyde-fixed, paraffin embedded (rat adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Inhibin Alpha) Polyclonal Antibody, Unconjugated (bs-10237R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.$

Paraformaldehyde-fixed, paraffin embedded (rat adrenal gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (Inhibin Alpha) Polyclonal Antibody, Unconjugated (bs-10237R) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

$Lane 1: Mouse Ovary tissue lysates; Lane 2: Mouse Placenta tissue lysates; Lane 3: Mouse Testis tissue lysates; Lane 4: Mouse Adrenal gland tissue lysates; Lane 5: Human HepG2 cell lysates; Lane 6: Human K562 cell lysates; Lane 7: Human MCF-7 cell lysates; Lane 8: Human Hela cell lysates; Lane 9: Human SH-SY5Y cell lysates probed with Inhibin Alpha Polyclonal Antibody, Unconjugated (bs-10237R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Ovary tissue lysates; Lane 2: Mouse Placenta tissue lysates; Lane 3: Mouse Testis tissue lysates; Lane 4: Mouse Adrenal gland tissue lysates; Lane 5: Human HepG2 cell lysates; Lane 6: Human K562 cell lysates; Lane 7: Human MCF-7 cell lysates; Lane 8: Human Hela cell lysates; Lane 9: Human SH-SY5Y cell lysates probed with Inhibin Alpha Polyclonal Antibody, Unconjugated (bs-10237R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
ELISA
IHC-P
IHC-F
IF(IHC-P)
IF(IHC-F)
IF(ICC)

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-10237R
Product Name	Inhibin Alpha Polyclonal Antibody
Applications	WB, ELISA, IHC-P, IHC-F, IF(IHC-P), IF(IHC-F), IF(ICC)
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from mouse Inhibin Alpha
Immunogen Range	281-366/366
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	16322
Swiss Prot	Q04997
Subcellular location	Secreted
Synonyms	AW555078; Inhibin alpha chain; Inha
Background	Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins. Inhibin deficient mice are viable but are acutely sensitive to development of gonadal sex-cord stromal tumors.
Application Dilution
WB	1:300-5000
ELISA	1:500-1000
IHC-P	1:200-400
IHC-F	1:100-500
IF(IHC-P)	1:50-200
IF(IHC-F)	1:50-200
IF(ICC)	1:50-200

Applications

Reactivity

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