CRHR1 Polyclonal Antibody, PE-Cy5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-10248R-PE-Cy5 |
| Product Name | CRHR1 Polyclonal Antibody, PE-Cy5 Conjugated |
| Applications |
WB
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human |
| Specifications | |
| Conjugation | PE-Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CRHR1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1394 |
| Swiss Prot | P34998 |
| Subcellular location | Cell membrane |
| Synonyms | Corticotropin-releasing factor receptor 1; CRHR1; CRF-R-1; CRF-R1; CRFR-1; CRFR |
| Background | G-protein coupled receptor for CRH (corticotropin-releasing factor) and UCN (urocortin). Has high affinity for CRH and UCN. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and down-stream effectors, such as adenylate cyclase. Promotes the activation of adenylate cyclase, leading to increased intracellular cAMP levels. Inhibits the activity of the calcium channel CACNA1H. Required for normal embryonic development of the adrenal gland and for normal hormonal responses to stress. Plays a role in the response to anxiogenic stimuli. |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is CRHR1 Polyclonal Antibody, PE-Cy5 Conjugated (bs-10248R-PE-Cy5) validated for, and at what dilution?
bs-10248R-PE-Cy5 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CRHR1 Polyclonal Antibody, PE-Cy5 Conjugated (bs-10248R-PE-Cy5) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human.
How should bs-10248R-PE-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CRHR1 Polyclonal Antibody, PE-Cy5 Conjugated (bs-10248R-PE-Cy5)?
KLH conjugated synthetic peptide derived from human CRHR1
What are the excitation and emission wavelengths of the PE-Cy5 conjugate (bs-10248R-PE-Cy5)?
PE-Cy5 on bs-10248R-PE-Cy5: excitation / emission 488nm/670nm.