phospho-Cofilin (Ser24) Polyclonal Antibody, PerCP Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-10252R-PerCP-100uL |
| Product Name | phospho-Cofilin (Ser24) Polyclonal Antibody, PerCP Conjugated |
| Applications |
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse, Dog, Cow, Chicken, GuineaPig |
| Specifications | |
| Conjugation | PerCP |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human CFL1/CFL2 around the phosphorylation site of Ser24 |
| Modification Site | Ser24 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1072 |
| Swiss Prot | p23528 |
| Subcellular location | Cytoplasm, Nucleus, Cell membrane |
| Synonyms | Cofilin/CFL1 Ser24; 18 kDa phosphoprotein; CFL 1; CFL; CFL1; Cofilin 1; Cofilin 1 non muscle; Cofilin non muscle isoform; p18; COF1_HUMAN. |
| Background | The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus. |
| Application Dilution | |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-Cofilin (Ser24) Polyclonal Antibody, PerCP Conjugated (bs-10252R-PerCP) validated for, and at what dilution?
bs-10252R-PerCP is validated for IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-Cofilin (Ser24) Polyclonal Antibody, PerCP Conjugated (bs-10252R-PerCP) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Dog, Cow, Chicken, GuineaPig.
How should bs-10252R-PerCP be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-Cofilin (Ser24) Polyclonal Antibody, PerCP Conjugated (bs-10252R-PerCP)?
KLH conjugated synthetic phosphopeptide derived from human CFL1/CFL2 around the phosphorylation site of Ser24
What are the excitation and emission wavelengths of the PerCP conjugate (bs-10252R-PerCP)?
PerCP on bs-10252R-PerCP: excitation / emission 482nm/677nm.