phospho-VAV3 (Tyr141) Polyclonal Antibody, PerCP-Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-10271R-PerCP-Cy5.5-100uL |
| Product Name | phospho-VAV3 (Tyr141) Polyclonal Antibody, PerCP-Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
IF(IHC-F)
IF(ICC)
|
| Reactivity | Mouse, Rat |
| Predicted Reactivity | Human, Cow, Sheep, Horse |
| Specifications | |
| Conjugation | PerCP-Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human VAV3 around the phosphorylation site of Tyr141 |
| Modification Site | Tyr141 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 10451 |
| Subcellular location | Cytoplasm, Extracellular matrix |
| Synonyms | VAV3phospho Y141; Guanine nucleotide exchange factor VAV3; Protein vav 3; Protein vav3; VAV 3; Vav 3 guanine nucleotide exchange factor; VAV 3 oncogene; VAV 3 protein; VAV-3; Vav3; VAV3 oncogene; VAV3 protein; VAV3_HUMAN. |
| Background | The Vav family of Rho guanine nucleotide exchange factors (GEFs) orchestrate signaling events following lymphocyte antigen receptor activation. Vav3, like Vav (also known as Vav1 or p95Vav), undergoes tyrosine phosphorylation downstream of T cell receptor cross-linkage, and subsequently interacts with 2 adaptor molecules, SLP76 and 3BP2. Following these events, however, the paths of Vav and Vav3 diverge; Vav affects IL-2 promotor activity, while Vav3 impacts gene transcription linked to serum response element (SRE). Furthermore, Vav3 expression follows a cell cycle-dependent pattern, with transient upregulation occuring during mitosis. Encforced Vav3 expression leads to the appearance of multinucleate cells, implicating a role for Vav3 in the control of cytokinesis. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
| IF(IHC-F) | 1:50-200 |
| IF(ICC) | 1:50-200 |
Frequently asked questions
Which applications is phospho-VAV3 (Tyr141) Polyclonal Antibody, PerCP-Cy5.5 Conjugated (BS-10271R-PERCP-CY5-5) validated for, and at what dilution?
BS-10271R-PERCP-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does phospho-VAV3 (Tyr141) Polyclonal Antibody, PerCP-Cy5.5 Conjugated (BS-10271R-PERCP-CY5-5) react with?
Tested reactivity: Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Cow, Sheep, Horse.
How should BS-10271R-PERCP-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise phospho-VAV3 (Tyr141) Polyclonal Antibody, PerCP-Cy5.5 Conjugated (BS-10271R-PERCP-CY5-5)?
KLH conjugated synthetic phosphopeptide derived from human VAV3 around the phosphorylation site of Tyr141
What are the excitation and emission wavelengths of the PerCP-Cy5.5 conjugate (BS-10271R-PERCP-CY5-5)?
PerCP-Cy5.5 on BS-10271R-PERCP-CY5-5: excitation / emission 482nm/694nm.