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Cytokeratin 19 Polyclonal Antibody, AbBy Fluor-350 Conjugated

Applications

  • WB
  • FCM
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human

Predicted Reactivity

  • Mouse
  • Rat
  • Dog
  • Cow
  • Pig
  • Horse
  • Chicken
  • Rabbit
Overview
Catalog # bs-1028R-BF350
Product Name Cytokeratin 19 Polyclonal Antibody, AbBy Fluor-350 Conjugated
Applications
WB FCM IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human
Predicted Reactivity Mouse, Rat, Dog, Cow, Pig, Horse, Chicken, Rabbit
Specifications
Conjugation AbBy Fluor-350
Host Rabbit
Source KLH conjugated synthetic peptide derived from human CK19
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 3880
Swiss Prot P08727
Subcellular location Cytoplasm
Synonyms Keratin, type I cytoskeletal 19; KRT19; Cytokeratin-19; CK-19; Keratin-19; K19
Background Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
Application Dilution
WB 1:300-5000
FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is Cytokeratin 19 Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-1028R-BF350) validated for, and at what dilution?

bs-1028R-BF350 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Cytokeratin 19 Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-1028R-BF350) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Pig, Horse, Chicken, Rabbit.

How should bs-1028R-BF350 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Cytokeratin 19 Polyclonal Antibody, AbBy Fluor-350 Conjugated (bs-1028R-BF350)?

KLH conjugated synthetic peptide derived from human CK19

What are the excitation and emission wavelengths of the AbBy Fluor-350 conjugate (bs-1028R-BF350)?

AbBy Fluor-350 on bs-1028R-BF350: excitation / emission 346nm/442nm.