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TBCEL Polyclonal Antibody, Cy5 Conjugated

Applications

  • WB
  • IF(IHC-P)
  • IF(IHC-F)
  • IF(ICC)

Reactivity

  • Human
  • Mouse
  • Rat

Predicted Reactivity

  • Dog
  • Cow
  • Sheep
  • Pig
  • Horse
  • Chicken
Overview
Catalog # bs-10292R-Cy5-100uL
Product Name TBCEL Polyclonal Antibody, Cy5 Conjugated
Applications
WB IF(IHC-P) IF(IHC-F) IF(ICC)
Reactivity Human, Mouse, Rat
Predicted Reactivity Dog, Cow, Sheep, Pig, Horse, Chicken
Specifications
Conjugation Cy5
Host Rabbit
Source KLH conjugated synthetic peptide derived from human TBCEL
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 219899
Swiss Prot Q5QJ74
Subcellular location Cytoplasm
Synonyms EL; Leucine-rich repeat-containing protein 35; LRRC35; TBCEL; TBCEL_HUMAN; Tubulin folding cofactor E-like; Tubulin-specific chaperone cofactor E-like protein.
Background Acts as a regulator of tubulin stability.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Frequently asked questions

Which applications is TBCEL Polyclonal Antibody, Cy5 Conjugated (bs-10292R-Cy5) validated for, and at what dilution?

bs-10292R-Cy5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200); IF(IHC-F) (1:50-200); IF(ICC) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does TBCEL Polyclonal Antibody, Cy5 Conjugated (bs-10292R-Cy5) react with?

Tested reactivity: Human, Mouse, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Dog, Cow, Sheep, Pig, Horse, Chicken.

How should bs-10292R-Cy5 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise TBCEL Polyclonal Antibody, Cy5 Conjugated (bs-10292R-Cy5)?

KLH conjugated synthetic peptide derived from human TBCEL

What are the excitation and emission wavelengths of the Cy5 conjugate (bs-10292R-Cy5)?

Cy5 on bs-10292R-Cy5: excitation / emission 625,650nm/670nm.